Use of the following instrumentation was at university, land-based laboratories and aboard research vessels.
Fields of Research
Biological Oceanography: Collection, identification, classification, testing, culturing and rearing of marine flora and fauna. Dissolved oxygen content analysis by the Winkler Titration Method. Use of chlorophyll rig, phytoplankton and zooplankton nets, and various trawls. Preservation of organisms in formalin and alcohol. Extensive use of compound and observation microscopes.
Chemical Oceanography: Seawater analysis of the following elements, compounds or properties and their respective instrumentation or methods used to analyze them:
Chlorosity, Chlorinity and Salinity - Mohr Volumetric, and Volhard (Potentiometric) Methods and Stoichiometry.
Salinity - Inductive and YSI portable Salinometers.
Hydrogen Ion Concentration - Electrometric (Beckman Expandomatic Meter).
Alkalinity - Titration with strong acid and stoichiometry using pH and Chlorinity results.
Carbon Dioxide - Van Slyke Gas Analysis Method.
Reactive Silicate - Nessler Tubes (a Colorimetric Method).
Phosphate and Reactive Nitrate - Nessler Tubes and Atomic Absorption Spectrophotometry (Beckman Du Spectrophotometer Model 2400).
Calcium - Flame Emission Photometry (Hitachi Flame Photometer fitted with a Phototube Multiplier No. 4300, Modified Flame Emission Attachment No. 9220 and a filter added to the Monochromator System).
Deep Sea Reversing Thermometers. Submarine Photometer. Transmissometer. NIO (National Institute of Oceanography) and Nansen Bottles. Use of sextant, Loran, Secchi Disks, Anemometers and assorted hydrowire hardware.
Chemical analysis of substrates. Precision Depth Recorder Analyses. Use of various Grab and Core samplers.
Researcher - Aquatic Research Institute, Port of Stockton, 15th and F Port Roads. Mailing address P.O. Box 648, Stockton, CA 95201. Dr. Robert R. Rofen - Director, (209) 466-5324. Accountable for the collection (SCUBA) and classification of marine invertebrates, and rearing of the institutes freshwater and marine life. 1966-68.
Staff Research Member - University of California, San Diego, Marine Life Research Group, Scripps Institution of Oceanography, La Jolla, CA. Dissolved Oxygen Titration Analysis. Published UC-SIO data report CalCOFI (California Cooperative Fisheries Investigation) Research Vessel New Horizon. 1984.
Academic Work Experience
Computers: Basic and Fortran IV on RSTS. Microsoft Windows, Basic and Graphics on microprocessors.
Inorganic Chemistry: Classical Quantitative Analysis - Gravimetric Analysis of Soluble Samples of: Silver Chloride for % Chloride; Barium Sulfate for % Sulfate. Titrimetric Analysis of Soluble Samples of: Unknown for % Chloride, Soda Ash for % Sodium Carbonate, Sodium Oxalate for % Oxalate. Titrimetric Analysis of Copper in Brass for % Copper. Volumetric Analysis of Iron Ore Sample for % Iron. Nonclassical Quantitative Analysis - Use of pH meters with various types of electrodes, for example, Potentiometric Analysis of: A Chloride-Iodide mixture, a Carbonate-Bicarbonate mixture, Iron (II) with Cerium (IV); and Conductometric Titrations: Acid-Base, and Precipitation. Electrogravimetric Analysis of % Copper in a Copper Sulfate solution. Analysis of Calcium by Atomic Absorption Spectrophotometry (a Perkin Elmer Model 103 with a Photoemission Attachment).
Organic Chemistry: Synthesis and Purification of Acetanilide. Fractional Distillation and Gas Chromatography of Cyclohexane and Toluene. Identification of unknown Hydrocarbons. Free Radical Chlorination. Dehydration of Methylcyclohexanol. Synthesis of Benzoic Acid by the Grignard Reaction. Identification of Unknown Aldehydes, Ketones and Amines. Use of Friedel Crafts Reaction to Alkylate the Aromatic (Benzene) Ring.
Comparative Anatomy and Physiology: Measurement of Osmolality of organisms bodily fluids by Vapor Pressure Osmometer and/or Freezing Point Depression compared with Melting Point. Flame Emission Photometry to find organisms Sodium concentration. Oxygen consumption by the Winkler Titration Method. Loading and Unloading of Oxygen by Respiratory Pigments, i.e., Absorption Spectra of Blood Pigments evaluated by Spectronic 20 (Bausch & Lomb) Spectrophotometer. Measure of Myocardial Action Potential (Contractions) by Wick Electrode, Amplifier and Oscilloscope. Q-10 (the change in Biological Systems) determined by Physiograph. LD 50 (Lethal Dose) and LCT (Lethal Concentration times Time) experiments. Use of Oxygen Meters.